Meet the Sanger Sequencing team!

Welcome back dear readers! Today I would like to introduce to you the people involved in one of our core services. Almost all of you know and hopefully also use 😉 our Sanger DNA Sequencing service. To make sure that we are able to process and sequence your samples in a short time, a well-organized, well-equipped and well-trained team is needed. The Sanger Sequencing team is by far the biggest team at inqaba biotec. This is on the one hand due to our goal to provide not only a simple sequencing service, but also Primer design, DNA isolation and analysis if requested. On the other hand this team is so big, because many interns receive their molecular biology training in the Sequencing lab, but they also perform most of the important hands on work.

inqaba biotec Sanger Sequencing Team (left to right): Tshidi, Lebo, Stella, Christiaan, Phodzo, Lerato, Mischa, Tinyiko, Iggy, Becky, Hazel

The man who holds everything together is Christiaan. He started at inqaba biotec 9 years ago as a technician. Back then, the sequencing team consisted of only 3 members, including him. Since 2008, Christiaan has been inqaba biotec’s Production Manager. He is responsible for all services and production and providing a myriad of services. His tasks include project design, sequence analysis, trouble shooting and technical support. Besides his busy work he is doing a PhD in genetics and in his rare leisure time he plays the piano and cello.

Christiaan shares the office with Mischa and Lerato. Mischa is the Senior Analyst for our animal genetics service. She is overseeing all the analysis, research and development involved in the animal genetics service. Mischa has an MSc in Biotechnology from University of Pretoria and will start her PhD in Animal Genetics next year. She is very happy about her very recent marriage, but also about her job that she describes as diverse, challenging and stimulating. Lerato also works as Senior analyst. She has an Honours degree in Biotechnology from University of Pretoria and currently studying towards her MSc. She started two years ago and her tasks include mainly the sample organisation and data analysis in both sequencing and animal genetics. In urgent situations, she also helps out in the lab. Lerato’s personal life also just went a step further… She just had a baby and is now a happy mother.

The person in charge of the laboratory is Stella. Stella studied Biotechnology at Vaal University of Technology and started at inqaba biotec in 2007 as an intern. Afterwards she worked as lab technician for 7 years and has been our Sequencing Lab Manager for 1 year now. So Stella has been here a long time, gained a lot of experience and stays calm also in busy/hectic times. She trains the interns and makes sure that all the samples get processed according to the protocol and in time.

meet_seq_lab1

We receive up to 600 samples per day, which all are processed immediately. 600 is a big number and demands for a lot of bench work. Luckily, in the sequencing lab we have at the moment 4 interns, Tinyiko, Hazel, Tshidi and Lebo, and 2 technicians, Phodzo and Iggy. We also have 2 night shift technicians, Johan who has an Msc in Biotechnology from University of Pretoria and Dewald who is studying towards his BSc in Biotechnology. They perform all the DNA extraction, purification and quality control experiments to assure that the DNA is ready for the sequencing. Tinyiko, Tshidi, Lebo, Phodzo and Iggy all studied Biotechnology, Hazel studied Genetics and Microbiology. Iggy mentioned that their team spirit is very good at work. They became friends, like to go out together and explore different foods.

After the successful DNA preparation, the actual sequencing can finally be performed. At inqaba biotec we have two ABI 3500XL genetic analyzers that are mainly run by Becky. Becky comes originally from Congo and came to South Africa for her Biotechnology studies. She started at inqaba biotec 4 years ago as an intern (as well!) and works now as lab technician. Becky performs the final steps towards the sequencing reaction, but also does genotyping. She likes reading, cooking and baking.

My personal impression of the sequencing team is that they are well-organized, enjoy their work and therefore manage every challenge with high quality and within a short time.

meet_seq_lab2

 

3 thoughts on “Meet the Sanger Sequencing team!”

  1. Auguste Nebo says:

    Hi. I am Auguste Nebo, in Cameroon at the university of Buea. Your research team is quite big and made of qualified personnel. I like that team spirit and the enthusiasm the younger ones show as you give them opportunities to have their internships there; and I look forward to working with you.
    I am currently carrying out my Msc. research work in molecular biotechnology and I want to do some sequencing work in order to identify fungi. I will like to know what are the things required for me to send my samples there, the cost of the work and also what are the different analyses you guys carry out with DNA sequences.
    Thanks in advance and hope to get from you soon.

  2. Christiaan Labuschagne says:

    Dear Auguste Nebo

    Thank you for your comment and interest in our services.

    We offer Fungal ID via ITS or EF as a service on pure cultures. Pricing would depend on the number of samples.
    The service includes DNA extraction, PCR of the relevant target, sequencing in both directions, assembly and editing of the data and a BLAST search with final report.

    We try and keep our services as flexible as possible and can trailer make the service to which ever starting material you wish to submit.

    Please contact me on my email christiaan.labuschagne@inqababiotec.co.za so we can put together a suitable package for you.

    All the Best
    Christiaan.

  3. Pule Shabalala says:

    Good day Nkohla,

    Thank you for your interest in joining the inqaba team.

    Kindly forward us your CV and academic record to info@inqababiotec.co.za. Should any vacancies be available, the relevant department manager will be in contact with you.

    All the best for your studies. Looking forward to hearing from you soon.

    Regards,
    Inqaba Team

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Contact Details

Southern Africa

  • Inqaba Biotechnical Industries (Pty) Ltd.
  • PO Box 14356, Hatfield 0028
  • Pretoria, South Africa

  • Tel: +27 12 343 5829
  • Fax: +27 86 677 8409
  • E-mail: info@inqababiotec.co.za

East Africa

  • inqaba biotec East Africa Ltd.
  • PO Box 1846
  • Nairobi 00606, Kenya

  • Tel: +254 735 370 693
  • Fax: +27 86 677 8409
  • E-mail: info@inqababiotec.co.ke

West Africa

  • inqaba biotec West Africa Ltd.
  • PMB 5320, Oyo Road, Ibadan 200001
  • Oyo State, Nigeria

  • Tel: +‎234 805 882 7272
  • Fax: +27 86 677 8409
  • E-mail: info@inqababiotec.ng