DNA Sequencing

DNA sequencing is becoming increasingly important in all disciplines of molecular biology, as well as in applied fields such as diagnostics or forensic research. The speed at which one obtains the results is therefore a critical factor in the efficiency of genomic research and diagnostic work.

In 2007, inqaba biotec upgraded the sequencing laboratory with the acquisition of a new ABI 3130XL sequencer. This has already decreased our turnaround time and improved the quality of the generated sequences. Geospiza Finch Suite, our new web based sequencing tracking and retrieval service, is making DNA sequence generation and data handling simpler, faster and more cost effective.

inqaba biotec offers tailor-made sequencing services; from a single reaction up to the sequencing of whole genomes. We offer a range of services ranging from simple lane reactions, giving you maximum control, to our premium service where your job ends with growing colonies containing the correct fragment.

Services

Simple Lane

Steps performed by inqaba biotec:
- Purification of the sequence reaction supplied by the customer
- Run on the ABI 3130XL Sequencer
No repeats

R 46 (excl Vat) per sequence

Please submit:
- Sequence reaction for purification or as cleaned-up sample and protected from light

Basic Service

Steps performed by inqaba biotec:
- Sequencing reaction of purified, quantified and concentration adjusted samples supplied by the customer.
- Run on the ABI 3130XL Sequencer
No repeat; template and primers need to be submitted at the correct concentration

R 75 (excl Vat) per sequence

Please submit:
- Purified PCR products at 10 ng/ul (for 100 to 500 bp amplicon size), 20 ng/ul (500 to 1000 bp for amplicon size) and an additional 10 ng/ul for every additional 500 bp in amplicon size
- Plasmid DNA at 100 ng/ul for plasmids up to 5kb in size and an additional 20 ng/ul for every additional 1 kb in plasmid size.
- The submitted template volume should be at least 10 ul plus 3 ul per additional sequence reaction with the same template.
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume supplied in separate tubes

Full Service

Steps performed by inqaba biotec:
- Purification and quantification of template
- Sequencing reaction
- Run on ABI 3130XL sequencer

No repeat

R 92 (excl Vat) per sequence

Please submit:
- Unpurified PCR products (20 ul of which 2 ul gave a strong and single band on an agarose gel)
- Unpurified plasmid preps or clones on plates (one clone per plate).
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume in separate tubes

Premium Service

Steps performed by inqaba biotec:
- Purification and quantification of template
- Sequencing reaction
- Optimisation, up to 3 x sequencing runs

R 160 (excl Vat) per sequence

Please submit:
- Unpurified PCR products (20 ul of which 2 ul gave a strong and single band on an agarose gel)
- Unpurified plasmid preps or clones on plates (one clone per plate).
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume in separate tubes

Additional Service

Priority
- R 20 (excl Vat) per sequence:
- Simple service within 24 hours,
- Basic service within 36 hours,
- Full and Premium within 48 hours after receipt of samples.
- If sequences are delivered after the deadline, priority surcharge will not be charged.

Sequence Editing:
- R 40 (excl Vat) per sequence read
- Both strands need to be sequenced

Sequence primer to be synthesized
- R 80 (excl Vat)

PCR amplification from gDNA
- R 100 (excl Vat) per amplification

Genomic DNA isolation
- R 100 (excl Vat) per isolation

Pick-up samples
- South Africa's main centres - R 30 (excl VAT)
- South Africa's outlaying centres - R 50 (excl VAT)
- Pretoria - R 20 (excl VAT)

Discounts

25 to 49 sequences of any services
- 5 % discount

55 to 99 sequences of any services
- 7.5 % discount

100 to 249 sequences of any services
- 10 % discount

over 250 sequences of any services
- 15 % discount

Sample Requirements

Samples and primers should be submitted in well marked tubes and should be well protected for shipment. They do not need to be cooled.

We keep stocks of standard primers like T7, T3, Sp6, M13 primers, T7 terminator, and, therefore, they do not have to be supplied by the customers.

Sequencing Tools:

Geospiza’s FinchTV

Geospiza’s FinchTV is a popular way to view DNA sequence traces on Linux, Mac OSX, Windows, and Solaris. FinchTV started as the only chromatogram viewer that can display an entire trace in a scalable multi-pane view. It leads the way with raw data views, BLAST searching and the ability to reverse complement sequences and traces.

Download FinchTV here

CLC Bio – The World’s leading bioinformatics solutions

CLC bio provides bioinformatics solutions within software, high-performance computing, consulting, and education to pharmaceutical andbiotechnology companies, as well as hospitals and universities all over the world; thereby facilitating research into genetically determined diseases, drug development, molecular diagnostics, etc. Furthermore CLC bio's internationally renowned scientists can help develop custom solutions based on individual preferences, requirements, and workflows.