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DNA sequencing is becoming increasingly important in all disciplines of molecular biology, as well as in applied fields such as diagnostics or forensic research. The speed at which one obtains the results is therefore a critical factor in the efficiency of genomic research and diagnostic work. In 2007, inqaba biotec upgraded the sequencing laboratory with the acquisition of a new ABI 3130XL sequencer. This has already decreased our turnaround time and improved the quality of the generated sequences. Geospiza Finch Suite, our new web based sequencing tracking and retrieval service, is making DNA sequence generation and data handling simpler, faster and more cost effective. inqaba biotec offers tailor-made sequencing services; from a single reaction up to the sequencing of whole genomes. We offer a range of services ranging from simple lane reactions, giving you maximum control, to our premium service where your job ends with growing colonies containing the correct fragment.
Services- Steps performed by inqaba biotec:
- Purification of the sequence reaction supplied by the customer
- Run on the ABI 3130XL Sequencer
- No repeats
- R 46 (excl Vat) per sequence
- Please submit:
- Sequence reaction for purification or as cleaned-up sample and protected from light
- Steps performed by inqaba biotec:
- Sequencing reaction of purified, quantified and concentration adjusted samples supplied by the customer.
- Run on the ABI 3130XL Sequencer
- No repeat; template and primers need to be submitted at the correct concentration
- R 75 (excl Vat) per sequence
- Please submit:
- Purified PCR products at 10 ng/ul (for 100 to 500 bp amplicon size), 20 ng/ul (500 to 1000 bp for amplicon size) and an additional 10 ng/ul for every additional 500 bp in amplicon size
- Plasmid DNA at 100 ng/ul for plasmids up to 5kb in size and an additional 20 ng/ul for every additional 1 kb in plasmid size.
- The submitted template volume should be at least 10 ul plus 3 ul per additional sequence reaction with the same template.
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume supplied in separate tubes
- Steps performed by inqaba biotec:
- Purification and quantification of template
- Run on ABI 3130XL sequencer
- R 92 (excl Vat) per sequence
- Please submit:
- Unpurified PCR products (20 ul of which 2 ul gave a strong and single band on an agarose gel)
- Unpurified plasmid preps or clones on plates (one clone per plate).
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume in separate tubes
- Steps performed by inqaba biotec:
- Purification and quantification of template
- Optimisation, up to 3 x sequencing runs
- R 160 (excl Vat) per sequence
- Please submit:
- Unpurified PCR products (20 ul of which 2 ul gave a strong and single band on an agarose gel)
- Unpurified plasmid preps or clones on plates (one clone per plate).
- The sequencing primers should be at 2 uM = 2 pmole/ul concentration and at least 20 ul in volume in separate tubes
- Priority
- R 20 (excl Vat) per sequence:
- Simple service within 24 hours,
- Basic service within 36 hours,
- Full and Premium within 48 hours after receipt of samples.
- If sequences are delivered after the deadline, priority surcharge will not be charged.
- Sequence Editing:
- R 40 (excl Vat) per sequence read
- Both strands need to be sequenced
- Sequence primer to be synthesized
- PCR amplification from gDNA
- R 100 (excl Vat) per amplification
- Genomic DNA isolation
- R 100 (excl Vat) per isolation
- Pick-up samples
- South Africa's main centres - R 30 (excl VAT)
- South Africa's outlaying centres - R 50 (excl VAT)
- Pretoria - R 20 (excl VAT)
- 25 to 49 sequences of any services
- 55 to 99 sequences of any services
- 100 to 249 sequences of any services
- over 250 sequences of any services
Samples and primers should be submitted in well marked tubes and should be well protected for shipment. They do not need to be cooled. We keep stocks of standard primers like T7, T3, Sp6, M13 primers, T7 terminator, and, therefore, they do not have to be supplied by the customers.
Geospiza’s FinchTV is a popular way to view DNA sequence traces on Linux, Mac OSX, Windows, and Solaris. FinchTV started as the only chromatogram viewer that can display an entire trace in a scalable multi-pane view. It leads the way with raw data views, BLAST searching and the ability to reverse complement sequences and traces. Download FinchTV here
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CLC bio provides bioinformatics solutions within software, high-performance computing, consulting, and education to pharmaceutical andbiotechnology companies, as well as hospitals and universities all over the world; thereby facilitating research into genetically determined diseases, drug development, molecular diagnostics, etc. Furthermore CLC bio's internationally renowned scientists can help develop custom solutions based on individual preferences, requirements, and workflows.  Click here to visit the world's leading bioinformatics solution provider, CLC bio.
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