RNA interference (RNAi) is a relatively new biological tool; however it has revolutionized genetic studies. Gene function studies have become a reality, as with this technology we are able to activate or repress the expression of specific genes. The applications of this technology are very broad, and range from basic gene function studies to applied research which includes modelling of human diseases, drug screening, testing of gene-based therapies and loss of function studies.

The most common strategy used to perform silencing studies is based on introducing synthetic siRNA into cells or DNA vectors expressing short hairpin RNAs (shRNAs) or miRNAs. The studies have continued to become more successful as more advanced products, including the shRNAs and improved transfection reagents have become available.

At inqaba biotec, we have the complete solution to all of your RNAi project needs. Working with an experienced research lab, we assist scientists with the design and generation of shRNAs. Our service includes the custom design, cloning and sequencing of plasmid expression vectors which can be applied in mammalian gene knockdown applications.

The following consultation services are offered:

• Target site selection
• Target site validation
• Protocols for the cloning and/or transfection (in vivo and in vitro)

Our RNAi service is well rounded as we also work with one of the leading international companies specialising in RNAi; PolyPlus Transfection. INTERFERin™ is a new generation siRNA delivery reagent developed by PolyPlus Transfection, and it achieves high transfection efficiency and efficient gene splicing. One of the most attractive features of the INTERFERin™ is that you can use very low siRNA concentrations in a wide range of cells without affecting cell viability.

For a complete solution to all of your RNAi needs, please This e-mail address is being protected from spambots, you need JavaScript enabled to view it .