Massarray Genotyping

MassARRAY System for medium to high throughput SNP detection

inqaba biotec and Agena Bioscience have partnered to introduce the MassARRAY technology in sub-Saharan Africa. The Agena MassARRAY System uses a single base-extension or cleavage chemistry in combination with MALDI-TOF analysis to meet your SNP genotyping or methylation analysis needs. There are over 3000 peer-reviewed publications for which data was generated using the MassARRAY system.

The MassARRAY SNP Genotyping System offers these unmatched benefits:
• High call rate irrespective of organism.
• high SNP multiplex capability in a single well (up to 36 SNPs per plex)
• Low cost per SNP
• Ability to quickly and easily modify an existing panel
• High sample throughput:
• Applications beyond SNP genotyping: the MassARRAY System supports copy number variation and methylation analysis.

How the SNP Genotyping Works

The SNP Genotyping assay consists of an initial locus-specific PCR reaction, followed by single base extension using mass-modified dideoxynucleotide terminators of an oligonucleotide primer which anneals immediately upstream of the polymorphic site of interest. Using MALDI-TOF mass spectrometry, the distinct mass of the extended primer identifies the SNP allele.

Sample Requirements

For each SNP-plex reaction, we need 5-10µl of 50-100ng/µl genomic DNA if quantitated on nanodrop, 25-50ng/uL if quantitated with PicoGreen dsDNA quantitation assay. A260/A280 ratio should be between 1.7 and 2.0.

For a quotation or to submit samples, please complete the MassARRAY Request Form and e-mail to Aron.Abera(at)inqababiotec.co.za